Anti-phospho-NKCC1 Thr212/Thr217

Application

Research CategorySignaling

Western Blotting Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GMB43 and GBM5 cell lysate (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31).
Immunofluorescence Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GBM43 human tissiue (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31).

Research Sub CategorySignaling Neuroscience

Anti-phospho-NKCC1 Thr212/Thr217 is an antibody against phospho-NKCC1 for use in Western Blotting, Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Na-K-Cl cotransporter 1 (NKCC1) is a membrane protein coded by the SLC12A2 gene and functions in cellular sodium/potassium/chloride transport and homeostasis. It is widely distributed throughout the body and serves important functions, especially in liquid-secreting glands. Phosphorylation of Thr212/Thr217 in the intracellular N-terminal domain of NKCC1 is correlated with NKCC1 activation.

Immunogen

Linear peptide corresponding to human phospho-NKCC1 Thr212/Thr217.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in GMB43 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-NKCC1 Thr212/Thr217 in 10 µg of GMB43 cell lysate.

Specificity

Specific for mature form of phosphorylated NKCC1.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~250 kDa observed. Likely a dimer of mature form of NKCC1, NKCC1 is glycosylated differently in different cells, leading to uncharacterized band(s) in some lysates.